Pocket Guide to Clinical Microbiology
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Christopher D. Doern. Pocket Guide to Clinical Microbiology
Table of Contents
List of tables
List of Illustrations
Guide
Pages
POCKET GUIDE TO Clinical Microbiology
Preface
About the Author
SECTION 1. Taxonomic Classification of Medically Important Microorganisms
Taxonomic Classification of Bacteria
Taxonomic Classification of Human Viruses
Taxonomic Classification of Fungi
Taxonomic Classification of Parasitesa
SECTION 2. Indigenous and Pathogenic Microbes of Humans
Microbes Responsible for Human Disease. BONE AND JOINT INFECTIONS
Summary of Notifiable Infectious Diseases: United States, 2015a
SECTION 3. Specimen Collection and Transport
Virology: General Specimen Guidelines
Virology: Specific Specimen Guidelines. Blood
Bone marrow
CSF
Respiratory specimens (throat, nasopharyngeal swab, nasopharyngeal aspirate, nasal washings, and bronchoalveolar lavage [BAL]) specimens)
Urine
Feces
Eyes
Tissue
Genital specimens
Skin
SECTION 4. Bacterial Diagnosis
Microscopy. Acid-Fast Stain
Acridine Orange Stain
Auramine-Rhodamine Stain
Direct Fluorescent-Antibody Stain
Gram Stain
Spore Stain
Primary Plating Media: Bacteria. Ashdown Medium
Bacteroides Bile-Esculin (BBE) Agar
Bile-Esculin (Enterococcal Selective) Agar
Bismuth Sulfite Agar
Blood Agar
Bordet-Gengou Agar
Brain Heart Infusion Agar and Broth
Brilliant Green Agar
Brucella Agar and Broth
Buffered Charcoal-Yeast Extract (BCYE) Agar
Burkholderia cepacia Selective Agar (BCSA)
Campylobacter Selective Medium
Cefsulodin-lrgasan-Novobiocin (CIN) Agar
Chromogenic Media
Chocolate Agar
Chopped-Meat Broth
Colistin-Nalidixic Acid (CNA) Agar
Columbia Agar and Broth
Cycloserine-Cefoxitin-Egg Yolk-Fructose Agar (CCFA)
Cystine Tellurite Blood Agar
Egg Yolk Agar
Ellinghausen-McCullough-Johnson-Harris Medium
Enterococcosel agar
Eosin-Methylene Blue (EMB) Agar
Fletcher Medium
Gram-Negative (G N; Hajna) Broth
Haemophilus Test Medium (HTM) Agar and Broth
Hektoen Enteric Agar
Kanamycin-Vancomycin Laked Blood (LKV) Agar
LIM Broth
LoffIer Medium
MacConkey (MAC) Agar
MacConkey Agar with Sorbitol—see Sorbitol-MacConkey Agar. Mannitol Salt Agar
Martin-Lewis Agar
McBride Listeria agar
Mueller-Hinton Agar and Broth
New York City Agar
Oxidative-Fermentative Polymyxin B-Bacitracin-Lactose (OFPBL) Agar
Phenylethyl Alcohol (PEA) Blood Agar
Regan-Lowe Agar Medium
Salmonella-Shigella (SS) Agar
Schaedler’s Agar
Selenite Broth
Skirrow Brucella Medium
Sorbitol-MacConkey Agar
Streptococcus selective medium
StrepB Carrot Broth
Tetrathionate Broth, Hajna
Thayer-Martin (Modified) Agar
Thioglycolate Broth
Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar
Tinsdale Agar
Tryptic or Trypticase Soy Agar (TSA) and Broth (TSB)
Xylose-Lysine-Deoxycholate (XLD) Agar
Primary Plating Media: Mycobacteria. American Trudeau Society Medium
Dubos Broth (Dubos Tween albumin broth)
Lowenstein-Jensen (LJ) Medium
Lowenstein-Gruft Modification
Lowenstein-Jensen Medium, Mycobactosel Modification
Middlebrook 7H9 Broth
Middlebrook 7H10 Agar
Middlebrook 7H11 Agar
Middlebrook 7H11 Agar, Mitchison’s Modification
Middlebrook 7H13 Broth
Petragnani Medium
Specific Diagnostic Tests for Pathogen Detection. Aerobic Gram-Positive Cocci
Aerobic Gram-Positive Rods
Acid-Fast and Partially Acid-Fast Gram-Positive Rods
Aerobic Gram-Negative Cocci
Aerobic Gram-Negative Rods
Anaerobic Bacteria
Curved and Spiral-Shaped Bacteria
Mycoplasma spp. and Obligate Intracellular Bacteria
SECTION 5. Viral Diagnosis
RNA Viruses
DNA Viruses
Transmissible Spongiform
SECTION 6. Fungal Diagnosis
Mycology: Specimen Collection and Transport Guidelines. General Guidelines
Dematiaceous Fungi
Dermatophytes (Epidermophyton, Microsporum, and Trichophyton spp.)
Dimorphic Fungi (Blastomyces, Coccidioides, Histoplasma, Paracoccidioides, and Sporothrix spp.)
Eumycotic Mycetoma Agents
Moniliaceous Fungi
Pneumocystis (carinii) jiroveci
Yeast
Microscopy. Acridine Orange Stain
Calcofluor White Stain
Fluorescent-Antibody Stain
Giemsa Stain
Gram Stain
India Ink Stain (Nigrosin)
Kinyoun Stain
Potassium Hydroxide (KOH)
Toluidine Blue-0 Stain
Primary Plating Media. Birdseed Agar
Brain Heart Infusion Agar (BHI)
CHROMagar Candida
Dermatophyte Test Medium (DTM)
Inhibitory Mold Agar (IMA)
Mycosel (Mycobiotic) Agar
Sabouraud Agar-Brain Heart Infusion (SABHI)
Sabouraud Dextrose Agar (SDA)
Yeast Extract-Phosphate Agar
Specific Diagnostic Tests
Biomarkers of Invasive Fungal Infection
SECTION 7. Parasitic Diagnosis
Acid-Fast Trichrome Chromotrope Stain
Calcofluor White Stain
Delafield’s Hematoxylin Stain
Direct Fluorescent-Antibody Stain
Giemsa Stain
Iron Hematoxylin Stain
Lugol’s Iodine Stain
Modified Acid-Fast Stain
Modified Acid-Fast Stains (Weber Green, Ryan Blue)
Trichrome Stain
Wright’s Stain
Specific Diagnostic Tests. Free-Living Amebae
Intestinal and Urogenital Protozoa
Blood and Tissue Protozoa
Microsporidia
Helminths: Nematodes
Helminths: Trematodes
Helminths: Cestodes
SECTION 8. Vaccines, Susceptibility Testing, and Methods of Organism Identification
Index. A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
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Double-stranded, enveloped RNA viruses
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