Pocket Guide to Clinical Microbiology

Pocket Guide to Clinical Microbiology
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Quick reference to clinical microbiology If you work in the clinical laboratory, this pocket guide will help you confidently identify most organisms you could encounter. This useful updated edition continues to present valuable quick-reference information to the clinical microbiology community in a small package. Along with specifics on pathogenic microorganisms, there is updated information on effectively using essential molecular diagnostic techniques for today's challenges. You will find guidance on: MALDI-TOF MS performance for individual bacteria, mycobacteria, and fungi Nucleic acid amplification testing/PCR and help interpreting genetic sequencing results Susceptibility testing, with methods and interpretive criteria for most organism/antibiotic combinations Antimicrobial resistance mechanisms and resistance profiles for common organisms

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Christopher D. Doern. Pocket Guide to Clinical Microbiology

Table of Contents

List of tables

List of Illustrations

Guide

Pages

POCKET GUIDE TO Clinical Microbiology

Preface

About the Author

SECTION 1. Taxonomic Classification of Medically Important Microorganisms

Taxonomic Classification of Bacteria

Taxonomic Classification of Human Viruses

Taxonomic Classification of Fungi

Taxonomic Classification of Parasitesa

SECTION 2. Indigenous and Pathogenic Microbes of Humans

Microbes Responsible for Human Disease. BONE AND JOINT INFECTIONS

Summary of Notifiable Infectious Diseases: United States, 2015a

SECTION 3. Specimen Collection and Transport

Virology: General Specimen Guidelines

Virology: Specific Specimen Guidelines. Blood

Bone marrow

CSF

Respiratory specimens (throat, nasopharyngeal swab, nasopharyngeal aspirate, nasal washings, and bronchoalveolar lavage [BAL]) specimens)

Urine

Feces

Eyes

Tissue

Genital specimens

Skin

SECTION 4. Bacterial Diagnosis

Microscopy. Acid-Fast Stain

Acridine Orange Stain

Auramine-Rhodamine Stain

Direct Fluorescent-Antibody Stain

Gram Stain

Spore Stain

Primary Plating Media: Bacteria. Ashdown Medium

Bacteroides Bile-Esculin (BBE) Agar

Bile-Esculin (Enterococcal Selective) Agar

Bismuth Sulfite Agar

Blood Agar

Bordet-Gengou Agar

Brain Heart Infusion Agar and Broth

Brilliant Green Agar

Brucella Agar and Broth

Buffered Charcoal-Yeast Extract (BCYE) Agar

Burkholderia cepacia Selective Agar (BCSA)

Campylobacter Selective Medium

Cefsulodin-lrgasan-Novobiocin (CIN) Agar

Chromogenic Media

Chocolate Agar

Chopped-Meat Broth

Colistin-Nalidixic Acid (CNA) Agar

Columbia Agar and Broth

Cycloserine-Cefoxitin-Egg Yolk-Fructose Agar (CCFA)

Cystine Tellurite Blood Agar

Egg Yolk Agar

Ellinghausen-McCullough-Johnson-Harris Medium

Enterococcosel agar

Eosin-Methylene Blue (EMB) Agar

Fletcher Medium

Gram-Negative (G N; Hajna) Broth

Haemophilus Test Medium (HTM) Agar and Broth

Hektoen Enteric Agar

Kanamycin-Vancomycin Laked Blood (LKV) Agar

LIM Broth

LoffIer Medium

MacConkey (MAC) Agar

MacConkey Agar with Sorbitol—see Sorbitol-MacConkey Agar. Mannitol Salt Agar

Martin-Lewis Agar

McBride Listeria agar

Mueller-Hinton Agar and Broth

New York City Agar

Oxidative-Fermentative Polymyxin B-Bacitracin-Lactose (OFPBL) Agar

Phenylethyl Alcohol (PEA) Blood Agar

Regan-Lowe Agar Medium

Salmonella-Shigella (SS) Agar

Schaedler’s Agar

Selenite Broth

Skirrow Brucella Medium

Sorbitol-MacConkey Agar

Streptococcus selective medium

StrepB Carrot Broth

Tetrathionate Broth, Hajna

Thayer-Martin (Modified) Agar

Thioglycolate Broth

Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar

Tinsdale Agar

Tryptic or Trypticase Soy Agar (TSA) and Broth (TSB)

Xylose-Lysine-Deoxycholate (XLD) Agar

Primary Plating Media: Mycobacteria. American Trudeau Society Medium

Dubos Broth (Dubos Tween albumin broth)

Lowenstein-Jensen (LJ) Medium

Lowenstein-Gruft Modification

Lowenstein-Jensen Medium, Mycobactosel Modification

Middlebrook 7H9 Broth

Middlebrook 7H10 Agar

Middlebrook 7H11 Agar

Middlebrook 7H11 Agar, Mitchison’s Modification

Middlebrook 7H13 Broth

Petragnani Medium

Specific Diagnostic Tests for Pathogen Detection. Aerobic Gram-Positive Cocci

Aerobic Gram-Positive Rods

Acid-Fast and Partially Acid-Fast Gram-Positive Rods

Aerobic Gram-Negative Cocci

Aerobic Gram-Negative Rods

Anaerobic Bacteria

Curved and Spiral-Shaped Bacteria

Mycoplasma spp. and Obligate Intracellular Bacteria

SECTION 5. Viral Diagnosis

RNA Viruses

DNA Viruses

Transmissible Spongiform

SECTION 6. Fungal Diagnosis

Mycology: Specimen Collection and Transport Guidelines. General Guidelines

Dematiaceous Fungi

Dermatophytes (Epidermophyton, Microsporum, and Trichophyton spp.)

Dimorphic Fungi (Blastomyces, Coccidioides, Histoplasma, Paracoccidioides, and Sporothrix spp.)

Eumycotic Mycetoma Agents

Moniliaceous Fungi

Pneumocystis (carinii) jiroveci

Yeast

Microscopy. Acridine Orange Stain

Calcofluor White Stain

Fluorescent-Antibody Stain

Giemsa Stain

Gram Stain

India Ink Stain (Nigrosin)

Kinyoun Stain

Potassium Hydroxide (KOH)

Toluidine Blue-0 Stain

Primary Plating Media. Birdseed Agar

Brain Heart Infusion Agar (BHI)

CHROMagar Candida

Dermatophyte Test Medium (DTM)

Inhibitory Mold Agar (IMA)

Mycosel (Mycobiotic) Agar

Sabouraud Agar-Brain Heart Infusion (SABHI)

Sabouraud Dextrose Agar (SDA)

Yeast Extract-Phosphate Agar

Specific Diagnostic Tests

Biomarkers of Invasive Fungal Infection

SECTION 7. Parasitic Diagnosis

Acid-Fast Trichrome Chromotrope Stain

Calcofluor White Stain

Delafield’s Hematoxylin Stain

Direct Fluorescent-Antibody Stain

Giemsa Stain

Iron Hematoxylin Stain

Lugol’s Iodine Stain

Modified Acid-Fast Stain

Modified Acid-Fast Stains (Weber Green, Ryan Blue)

Trichrome Stain

Wright’s Stain

Specific Diagnostic Tests. Free-Living Amebae

Intestinal and Urogenital Protozoa

Blood and Tissue Protozoa

Microsporidia

Helminths: Nematodes

Helminths: Trematodes

Helminths: Cestodes

SECTION 8. Vaccines, Susceptibility Testing, and Methods of Organism Identification

Index. A

B

C

D

E

F

G

H

I

J

K

L

M

N

O

P

Q

R

S

T

U

V

W

X

Y

Z

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4th Edition

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Double-stranded, enveloped RNA viruses

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