Modern Techniques in Cytopathology
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Группа авторов. Modern Techniques in Cytopathology
Monographs in Clinical Cytology
Modern Techniques in Cytopathology
Contents
Preface
Acknowledgements
Disclosure Statement
References
The Interventional Cytopathologist and Ultrasound-Guided Fine-Needle Aspiration
Abstract
Training
US-Cytology Correlation
Credentialing
Equipment
Reporting
Looking Forward: Elastography
Disclosure Statement
References
Cell Blocks: Evolution, Modernization, and Assimilation into Emerging Technologies
Abstract
Histology versus Cytology
Cell Blocks: Overview
Cell Blocks: Advantages
Cell Blocks: Current Methods
Cell Blocks: Cellient Automated Cell Block System
Cell Blocks: Cellularity
Cell Blocks: Fixation and Ancillary Studies
Cell Blocks: Morphology
Cell Blocks: Evolution, Emerging, and Modern Techniques
Cell Blocks: Application to Modern and Alternative Techniques
Conclusion
References
Fluorescence in situ Hybridization in Cytopathology
Abstract
Specimen Processing and Interpretation
FISH Testing and Uses of Different Probes
Break-Apart Probes
Dual Color or Fusion Translocation Probes
Amplifications and Deletions
Applications in Clinical Testing Using Cytology Specimens
Non-Small Cell Lung Carcinoma
Salivary Gland Tumors
Soft Tissue Tumors
Hematolymphoid Proliferations
Pediatric Tumors and Round Blue Cell Tumors
Urothelial Carcinoma and Pancreatobiliary Adenocarcinoma
Conclusion
Acknowledgments
References
Next-Generation Sequencing in Cytopathology
Abstract
NGS Overview
NGS in Cytology
Specimen Cellularity
Specimen Preparation Type
Fixatives and Stains
DNA Yield and Input DNA
Other Preanalytical Factors
Applications of NGS in Cytology
Thyroid
Pancreas
Lung
Challenges and Future Directions
Disclosure Statement
References
Liquid Biopsy: Recent Advances in the Detection of Circulating Tumor Cells and Their Clinical Applications
Abstract
Cell-Free DNA
Pathogenesis of CTCs
Clinical Applications of CTCs (Liquid Biopsy)
Composition of Blood Cells and Numbers of CTCs
Half-Life of CTCs and Tumor Dormancy
Concept of Lineage Plasticity
Types of Platforms Used for the Detection of CTCs
Antigen-Dependent CTC Methods. Immunomagnetic Capture
Bead-Based Subtraction-Enrichment Strategies
Antigen-Independent CTC Methods. Enrichment-Free Platforms (“No Cell Left Behind”)
Fluorescent in situ Hybridization
Filtration Methods
Single-Cell Genomic Analyses of CTCs
Specific Organ Sites. Lung
Breast
Conclusion and Perspectives
Acknowledgements
Disclosure Statement
Appendix. Ficoll-Hypaque Procedure for Peripheral Blood
FISH Protocol
Pretreatment of Slides
Hybridization
Post-Hybridization Wash
CellSieve Method of Filtration
References
Computer-Assisted Pap Test Screening
Abstract
Pap Test Screening
Automated Screening Systems. Early Rescreening Systems
Primary Screening Systems
Emerging Screening Systems
Challenges and Opportunities
Digital Imaging Technology
The MAVARIC Trial
Workload Limits
Conclusion
Disclosure Statement
References
Telecytology for Rapid On-Site Evaluation
Abstract
Telecytology Platforms
Static Telecytology
Dynamic Real-Time Imaging
Whole-Slide Imaging
Hybrid Systems
Key Considerations for Clinical Practice
Intended Use Case
Pre-Implementation
Deployment
Post-Implementation
Pros and Cons. Advantages
Disadvantages
Published Experience
Conclusion
Disclosure Statement
References
Whole-Slide Imaging in Cytopathology
Abstract
Unique Challenges
Z-Stacking
Image Quality
Cytology Workflow
Imaging Applications
Primary Diagnosis
Education
Proficiency Testing
Conclusion
Disclosure Statement
References
Image Analysis in Cytopathology
Abstract
Image Analysis Methods
Quantitative Analysis
Artificial Intelligence
Challenging Image Datasets
Applications of Computational Cytology. Gynecological Cytopathology
Non-Gynecological Cytopathology
Conclusion
Disclosure Statement
References
Other Cytology Applications: Clinical Trials, Checkpoint Inhibitors, and Cytobanking
Abstract
Clinical Trials
ROSE
Immune Checkpoint Inhibitors
Cytobanking
Disclosure Statement
References
Author Index
Subject Index
Отрывок из книги
Modern Techniques in Cytopathology
Series Editor
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23Sung S, Crapanzano JP, DiBardino D, Swinarski D, Bulman WA, Saqi A. Molecular testing on endobronchial ultrasound (EBUS) fine needle aspirates (FNA): Impact of triage. Diagn Cytopathol. 2018 Feb;46(2):122-130.
24Kruger AM, Stevens MW, Kerley KJ, Carter CD: Comparison of the CellientTM automated cell block system and agar cell block method. Cytopathology 2014;25:381–388.
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