"Section Cutting and Staining" by Walter S. Colman. Published by Good Press. Good Press publishes a wide range of titles that encompasses every genre. From well-known classics & literary fiction and non-fiction to forgotten−or yet undiscovered gems−of world literature, we issue the books that need to be read. Each Good Press edition has been meticulously edited and formatted to boost readability for all e-readers and devices. Our goal is to produce eBooks that are user-friendly and accessible to everyone in a high-quality digital format.
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Walter S. Colman. Section Cutting and Staining
Section Cutting and Staining
Table of Contents
PREFACE TO THE SECOND EDITION
SECTION CUTTING AND STAINING
CHAPTER I
Apparatus Required
CHAPTER II
Hardening Processes
Special Hardening Reagents for Rapid Fixation in Order to Study Cell Structure
Decalcifying Fluids
CHAPTER III
Section Cutting
CHAPTER IV
Section Mounting
Mounting Media
CHAPTER V
General Staining Methods
CHAPTER VI
Special Staining Methods.—Special Methods for Staining the Nerve Centres
CHAPTER VII
Special Methods for Staining Micro-organisms and Blood
Methods of Examining Blood
Staining Methods
CHAPTER VIII
Injection of Blood Vessels
CHAPTER IX
Directions for Preparing Individual Tissues
BOOKS OF REFERENCE
INDEX
FOOTNOTES:
Отрывок из книги
Walter S. Colman
A practical introduction to histological methods for students and practitioners
.....
The fluid used must be one which will not itself injure the specimen, and which can be thoroughly removed by washing, so that it may not interfere with staining operations. The specimens should be kept while hardening in wide mouthed bottles, on the bottom of which a little cotton wool or tow has been laid. This allows the hardening fluid to come freely in contact with the under surface of the pieces of tissue, and prevents their being flattened against the hard glass bottom.
The hardening fluid requires changing occasionally. This should always be done at the end of twenty-four hours, in order to get rid of any deposit of blood, &c., that may have accumulated. Besides this, the tissue when placed in the fluid contained a good deal of water which will have diluted it and consequently an early change is desirable. Afterwards the fluid requires to be changed only as often as it becomes turbid, or any deposit occurs, usually about once a week.
