Читать книгу Analytical Food Microbiology - Ahmed E. Yousef - Страница 57

Pour‐plating involves dispensing a portion of the sample or its dilution (commonly 1 ml) into a standard Petri plate, adding molten agar medium (10–15 ml, at 48–50°C), mixing plate contents carefully, and letting the mixture solidify. Using this technique requires that molten agar media be prepared ahead of the sample preparation and held in a water bath set at ~50°C until poured. The molten medium could be prepared in bulk in Erlenmeyer flasks or partitioned in test tubes. In the former case, a skilled analyst can pour the agar into multiple plates at quantities suitable for the analysis. In the latter case, the agar quantity in each tube should be sufficient to prepare one plate.