Читать книгу Immunophenotyping for Haematologists - Barbara J. Bain, Irene Roberts - Страница 9

Immunohistochemistry predominantly employs a primary monoclonal antibody directed at the target antigen, followed by a secondary anti‐immunoglobulin antibody that is coupled to an enzyme; the enzyme can subsequently participate in an enzymatic reaction, producing a coloured product that can be visualised. The most frequently used technique is an immunoperoxidase reaction. For some purposes, for example, the detection of immunoglobulin components, polyclonal antisera may be preferred. Immunohistochemistry has an advantage over flow cytometry in that antigen expression can be related to cytological and histological features. Co‐expression of antigens can be studied by using two different enzymatic reactions (such as peroxidase and alkaline phosphatase) or by identifying the same cell population in serial sections of the tissue.