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Box 1.7 Aspects to be considered when determining the sample size A larger sample size is needed when there is:

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 high variability – use a pilot study or consult similar investigations in the literature to get a feel for the likely variability;

 a small difference or relationship or association to be detected – it is worth recognising that very small differences may not be important ecologically (e.g. a native plant may have more insect species than an introduced one, but if this difference is by only one or two common insects, it is unlikely to be of conservation importance);

 a requirement to subdivide the data for analysis (e.g. separate analysis of males and females would require similar appropriate sample sizes of both males and females).

See Krebs (1999), van Belle (2002), and various online calculators25 for further details of the different calculations that can be used to estimate sample sizes, depending on the intended statistical analysis technique to be used.

In surveys of community structure, it may be important to know that the majority of species in an area have been recorded at least once in your sample. In this case, species accumulation curves may help. At its simplest, this involves plotting the accumulated number of species against increasing sampling effort. Sampling effort is the number of sampling units (quadrats, pitfall traps, animals handled, hours of observations, sites surveyed, etc.). Box 1.8 illustrates the use of species accumulation curves in quadrat sampling (see Chapter 3). There are a variety of methods of modelling species accumulation curves (see Colwell et al. 2004 and Magurran 2004 for further information) and many standard software packages include routines for this (e.g. those obtained from Pisces Conservation).26

Practical Field Ecology

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