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Polymerase Chain Reaction Test for the SRY Gene

In addition to chromosome analysis and karyotyping, it is a common practice in horse cytogenetics laboratories to test each case for the presence or absence of the SRY gene in the Y chromosome. The procedure involves polymerase chain reaction (PCR) with SRY primers and the genomic DNA of the horse in question. The test is useful for cases of sex reversal where a sterile female horse has XY sex chromosomes, with or without the SRY gene. The PCR test can also discover female horses that are mosaic or chimeric for a cell line carrying the Y chromosome.