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Normograde Oviductal Flush (Laparotomy Approach) Technique

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Performed under general anesthesia.

 The mare is fasted for 24 hours prior to surgery.

 Antibiotics and flunixin meglumine (1.1 mg/kg IV, s.i.d.) are administered prior to surgery and for 3 days after surgery.

 The mare is placed under general anesthesia and in dorsal recumbency and a pelvic tilt apparatus applied to gain better access to the reproductive tract.

 The ventral abdomen is clipped free of hair.

 A standard surgical prep is made over the ventral abdomen.

 A 10 cm incision is made along the ventral midline just cranial to the udder.

 One ovary and uterine horn are exteriorized and examined for gross pathology.

 Doyen intestinal forceps are placed on the uterine horn 5 cm from the cranial tip to occlude the uterine horn.

 An 8.0 Fr balloon‐tipped catheter is passed into the infundibulum of the oviduct and down to the ampulla region and the cuff inflated with approximately 1.0–1.5 ml of air.

 20 ml of sterile saline with new methylene blue dye is slowly injected through the catheter as the catheter is held in the oviduct by the application of manual pressure proximal to the balloon.

 Patency of the oviduct may be confirmed by the techniques below:Injection and aspiration of 10–20 ml of sterile saline into the occluded cranial uterine horn to detect the presence of dye.Injection of 20 ml of air through the catheterized oviduct and listening for the sound of air “gurgling” through the UTJ into the uterine lumen.The advantage of performing these confirmation procedures is that additional flushing could be performed if the oviduct is not yet patent.Alternatively, an endoscopic examination of the uterine lumen could be used to visualize the dye. This could be accomplished by a second set of personnel during surgery while the horse is under general anesthesia.

 The procedures are repeated on the contralateral oviduct.

 The abdomen is closed in a routine manner.

Equine Reproductive Procedures

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