Читать книгу Modern Techniques in Cytopathology - Группа авторов - Страница 28

The effect on morphology by fixative (alcohol and formalin) and different cell block preparations has been examined. Similar to outcomes reported for cellularity and ancillary studies, these results also vary. In general, alcohol fixation is associated with shrinkage artifacts and better nuclear detail [12]. Some investigators have detected no significant difference between traditional methodology (e.g., HistoGel, plasma-thrombin) and CACBS [21, 28, 30], whilst others have noted a statistically significant improvement in the appearance of the cytoplasm, nucleoli, and chromatin in CACBS-prepared cell blocks [24, 29], attributable to its alcohol-based fixation [33].