Читать книгу Diatom Morphogenesis - Группа авторов - Страница 16

Toward the complete understanding of the 3D structure of a given diatom frustule, a comprehensive 3D model can be created from the data collected from different characterization techniques. This approach, which is designated as the 3D reconstruction of diatom frustules, can be used for different purposes but is mainly for computer modeling. Oncoming tools for the 3D reconstruction of diatom frustules are FIB-SEM [1.32, 1.42] and digital holographic microscopy (DHM) combined with SEM [1.30]. The combination of DHM and SEM or AFM might give the ability to model and visualize microscopic 3D objects with a high resolution in all directions [1.30]. Hildebrand et al. [1.32] introduced the ability for the 3D reconstruction of subcellular architecture using FIB-SEM with new insights into the architecture and synthesis process of both the siliceous and organic components inside the cell. Xing et al. [1.42] is an inspiring reference for the 3D reconstruction of diatom frustule using the 2D image series resulting from FIB-SEM.