Читать книгу Principles of Microbial Diversity - James W. Brown - Страница 47

For most sequences commonly used for phylogenetic analysis, there are specialized databases of pre-aligned sequences. There are several databases of SSU rRNAs and their alignments: the Ribosomal Database Project is a prime example and is specialized for phylogenetic analysis. As of this writing, the Ribosomal Database Project contains 1.3 million aligned SSU rRNA sequences and a suite of software tools to access these data, including a taxonomic browser that can be used to collect any desired aligned sequences for further analysis. Databases of this type are usually the best starting point from which to collect an initial data set.

Very often, however, there is additional useful information that is not yet available in these specialized databases. BLAST searches of general sequences databases (e.g., GenBank), most often through the National Center for Biotechnology Information website, often identify additional useful sequences.