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DIAGNOSTICS

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 Blood sampling is essential for diagnosis and monitoring of anemic reptiles.

 Patient’s size, condition, and venous access must all be considered.

 Venipuncture sites with minimal lymphatic contamination are preferred, to obtain a more representative sample of the peripheral blood.

 In chelonians, the jugular vein is the site with the least amount of lymphatic contamination.

 Alternatively, the brachial plexus and coccygeal veins can be sampled.

 The subcarapacial sinus tends to have the largest amount of lymphatic contamination.

 In critically ill, possibly anemic, reptiles, aim to collect a blood volume of no more than 0.5% of the body weight (0.5 ml/100 g body weight) so as to not exacerbate the anemic condition.

 EDTA can cause hemolysis and artifacts in most chelonian, so lithium heparin is the preferred anticoagulant.

Blackwell's Five-Minute Veterinary Consult: Reptile and Amphibian

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