Читать книгу Quantum Evolution: Life in the Multiverse - Johnjoe McFadden - Страница 22

Two years before the 1868 edition of The Origin of Species, an obscure monk working in an Augustinian monastery in Brünn had discovered and published the right answer to Darwin’s problem. Born into a peasant family in Silesia, Gregor Mendel joined the monastic life to escape poverty. In the monastery garden, Mendel crossed pea plants and carefully recorded the inheritance of parental traits – such as round or wrinkled peas – in those hybrids he generated. He demonstrated that, contrary to expectations, discrete characteristics such as the shape of the pea seed did not blend in crosses. Instead they bred true – were unchanged when they appeared in subsequent generations – though sometimes skipping a generation. To account for his peas, Mendel proposed that organisms contain within them discrete factors, passed unaltered from one generation to the next. We now call Mendel’s discrete factors, genes. This was the exactly the answer Darwin needed – genetic variation would not be lost during sexual reproduction but emerge, unscathed by its passage, in each generation.

Darwin died in 1882, completely unaware that the work that would rescue his seriously flagging theory was languishing in the Linnaean Society library, the very place his own theory had been triumphantly unveiled. Even more tragically, Mendel died in obscurity in 1884, his revolutionary work unknown or forgotten. It was not until 1900 that three botanists, Hugo de Vries, Carl Correns and Erik von Tschermak, independently rediscovered his experiments. Working on the inheritance of variation in plants, they were each finding evidence for discrete patterns of inheritance. Searching the literature for any related similar work, independently each came across brief references to Mendel’s publications and immediately realised their significance. Mendel was posthumously recognized as the father of modern genetics. Mendelian genetics went on to revolutionize twentieth-century biology and medicine.

The early twentieth century saw the fusion of Darwinian evolutionary theory (his original rather than the Revised Version) and Mendelian genetics in what has come to be known as the neo-Darwinian synthesis. In 1901 De Vries published the first volume of his Mutation Theory, in which he proposed that evolution occurs by discrete steps or mutations that were rare chance modifications of Mendelian genes. These mutations were the source of the variation for Darwinian natural selection and evolution. Evolutionary theory was, at last, complete.

But what were genes? What were they made of? How were they inherited? How were they modified? In 1901 nobody had any idea. The first real clue did not come until 1945 when the bacteriologist Oswald Aver/s experiments at New York’s Rockefeller Institute, demonstrated that genetic characteristics could be transferred from one bacterial cell to another, simply by transferring a chemical called deoxyribonucleic acid, or DNA. Avery purified DNA from some bacterial cells which produced a capsule (a slimy protective layer made of strings of sugars that surrounds the bacterial cells). He found that if he added it to cells that didn’t produce a capsule then some of them would be transformed into capsule-producing bacteria. It appeared that the genetic information, the gene for the capsule, was made of DNA and could be transferred from one bacterial cell to another simply by transferring the DNA chemical.

However, not everyone was convinced of the significance of Avery’s demonstration that DNA encoded bacterial slime. DNA was considered an unlikely vehicle for heritable information. Different species were assumed to have different genes but DNA isolated from different species appeared identical. The prevailing opinion was that genes were made of the protein. It was easy to show that different species had different proteins. Proteins contaminated all preparations of DNA, so many scientist’s believed that it was the contaminating proteins in Avery’s experiments that had transferred the genetic information. When in 1944, the quantum physicist Erwin Schrödinger (of whom much more later) published his book, What is Life?, he went along with the prevailing genes as proteins hypothesis.

However, in the early 1950s Alfred Hershey and Martha Chase’s experiments proved that genes were made of DNA. They demonstrated that when a virus infects a bacterial cell, it injects its DNA but not its protein into the host cell. After infection, the bacteria become transformed to make the bacteriophage proteins. So the genes encoding those proteins must have been injected with the bacteriophage DNA. Genes must be made of DNA.

DNA was quickly accepted as the genetic material, but it was unclear how genetic information was stored within it. The overall chemical composition of DNA was already known – it was composed of a simple sugar (deoxyribose), phosphate groups and roughly equal quantities of four types of nucleic acids, each made up of carbon, nitrogen and hydrogen atoms. But the profound problem remained – how do these chemicals store the information for the shape of your nose? This was answered by Watson and Crick’s structure.