Manual for laboratory classes in biological physics

Manual for laboratory classes in biological physics
Автор книги: id книги: 803657     Оценка: 0.0     Голосов: 0     Отзывы, комментарии: 0 192 руб.     (1,87$) Читать книгу Купить и скачать книгу Купить бумажную книгу Электронная книга Жанр: Медицина Правообладатель и/или издательство: КазНУ Дата публикации, год издания: 2016 Дата добавления в каталог КнигаЛит: ISBN: 978-601-04-2471-5 Скачать фрагмент в формате   fb2   fb2.zip Возрастное ограничение: 0+ Оглавление Отрывок из книги

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Описание книги

The methodical development for Laboratory Work in Biophysics is a set of teaching materials and guidelines for laboratory work in biophysics that has been prepared and held at the Al-Farabi Kazakh National University. This book is designed for a small biophysical workshop and a special workshop. The works presented here do not require complex and expensive equipment and can easily be reproduced in any university laboratory. The methodical development describes the main sections of Biophysics: thermodynamics of electrical conductivity in biological systems, bioelectric phenomena, photometric methods of biological system’s research, lasers in biology and medicine, and others. Self-help questions that were designed to further the understanding of the processes and phenomena observed during laboratory work can be found at the end of each chapter. This guide is intended for university students studying in the fields of biology, biotechnology, ecology, and medicine. Publishing in authorial release. Методические разработки предназначаются для малого биофизического практикума и специального практикума. Работы, представленные в данном руководстве, не требуют сложного и дорогого оборудования и могут легко быть воспроизведены в любой университетской лаборатории. В книге рассмотрены основные разделы биофизики: термодинамика, электропроводность биологических систем, биоэлектрические явления, фотометрические методы исследования биологических систем, лазеры в биологии и медицине, и др. Расположенные в конце каждой главы вопросы для самоконтроля способствуют более прочному и глубокому пониманию процессов и явлений, наблюдаемых в лабораторной работе. Настоящие разработки предназначаются для студентов, обучающихся по специальностям «Биология», «Биотехнология», «Экология», а также для студентов медицинских специальностей университетов. Издается в авторской редакции.

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Коллектив авторов. Manual for laboratory classes in biological physics

FOREWORD

SAFETY REQUIREMENTS DURING BIOPHYSICS WORKSHOPS

Chapter 1. THERMODYNAMICS

Laboratory work № 1. Determination of temperature coefficient and calculation of the activation energy of the frog’s heart

Laboratory work № 2. Determination of the temperature coefficient and calculation of the action energy of respiration of elodea plant branch

Chapter 2. WORKING PRINCIPLE OF PH-SENSOR

Laboratory work № 3. pH of various solutions and biological liquids

`Laboratory work № 4. Determination of pH of fermented milk products using a pH meter

Chapter 3. BUFFER PROPERTIES AND BUFFER CAPACITY OF BIOLOGICAL LIQUIDS

Laboratory work № 5. Definition of alkaline and acid buffers of serum blood

Laboratory work № 6. Buffer capacities of biological liquids

Chapter 4. THE OXYGEN CONTENT IN WATER

Laboratory work № 7. Determination of the oxygen content dissolved in water

Laboratory work № 8. Amperometric method of determining the rate of respiration of aquatic plants and yeast cells

Laboratory work № 9. Study of electrical parameters of the solutions and wash water of seeds

Chapter 5. PHOTOMETRIC METHODS OF BIOLOGICAL SYSTEM’S RESERCH

Laboratory work№ 10. Working principle of photoelectrocolorimeter

Laboratory work № 11. Measurement of optical density of different solutions

Laboratory work № 12. The method of vital staining of tissues

Laboratory work № 13. Studying of sorption of dyes by bio objects depending on the action of various physical factors

Laboratory work № 14. Water sorption studying by fabrics depending on action of various physical factors

Laboratory work № 15. Spectrophotometer

Chapter 6. OPTICAL PROPERTIES OF BLOOD

Laboratory work № 16. Effects of different chemicals on the light scattering and light absorption of hemoglobin solution

Laboratory work № 17. The study of photodynamical hemolysis of erythrocytes

Chapter 7. POLARIMETRY

Laboratory work № 18. Sugar concentration measurements by the polarimeter SU-4

Chapter 8. LASERS IN BIOLOGY AND MEDICINE

Laboratory work № 19. Working principle of laser

Laboratory work № 20. Study of the properties of natural light and artificial light sources

Chapter 9. ELECTROCONDUCTIVITY OF LIVING SYSTEMS

Laboratory work № 21. The study of the laws of the passage of an electric current through living tissue

Laboratory work № 22. Galvanizing. Electrophoresis of drugs

Chapter 10. BIOLOGICALLY ACTIVE POINTS OF HUMAN AND ANIMAL SKIN

Laboratory work № 23. The study of biophysical parameters of biologically active points of human skin

Chapter 11. ELECTROCARDIOGRAPHY

Laboratory work № 24. The study of human electrocardiogram during exercise

Laboratory work № 25. Construction of the electrical axis of the heart

Chapter 12. GALVANIC SKIN RESPONSE

Laboratory work № 26. The study of human’ galvanic skin response

APPLICATION

Textbook & Course Materials

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Before carrying out any laboratory work, students are required to undergo safety training. The students will also have to sign the control sheets that indicate completion of the training.

During labs, instruments that meet standard requirements and have proper technical specifications and normative technical documentation could be used. The equipment is checked directly by the instructor before the laboratory.

.....

1. Count the amount of gas bubbles at room temperature;

2. Count the amount of gas bubbles during the rise of the temperature by 10 °C;

.....

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