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After the use of cell therapy, some cells may be still not utilized. These cells should be preserved in order to repeat the procedure in the distant future.

To preserve your own cells, you need to place them in a special cryostorages. It is a duralumin or steel container, made like a type of thermos, with double walls, between which a vacuum is created. It is filled with liquid nitrogen having a temperature of minus 196⁰C (Figures 16a, 16b).

Before freezing, the cells are washed from the growth medium and kept in a protective solution that prevents them from damage at low temperature.

Cell suspension is poured into special sterile plastic vials, closed hermetically. These ampoules with cells inside are first subjected to gradual smooth cooling to minus 70—80⁰С, after which they are immersed in liquid nitrogen in the cryostorage, where they remain for many years and decades. Each of the cryovials is assigned a unique identification number, which is entered into a computer database. This number is strictly consistent with the person from whom the cells were taken. The database contains the following data: name and surname of the person, date of birth, blood characteristics, information about hereditary diseases, the date of taking a biopsy, some other information.

The database exists not only in the computer, but the information is also duplicated on CDs or USB flash drives which are stored in a dedicated place. This eliminates the loss of information. The cryopreservation itself is constantly under the supervision of the personnel on duty, it is excluded from the access of unauthorized persons, and all manipulations with containers are made only by carefully trained personnel who are personally responsible for the safety of cellular material.

In such a cryostorage the fibroblasts or other cells can be stored indefinitely, and if necessary, part of them can be extracted, thawed and used for cell therapy of the skin, organs or other purposes in the future.