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Microsporidia

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As mentioned above, microsporidia are the most difficult protozoa to diagnose (size range, 1 to 2.5 μm). Dissemination from the intestine to other body sites has been well documented. Modified trichrome stains have been developed specifically for detection of these organisms, since routine parasitology stains for fecal specimens are not very effective for microsporidial spores. Optical brightening agents such as Calcofluor are also recommended; although they are very sensitive, they are nonspecific. Currently, at least 14 species have been identified as human parasites. Genera include Brachiola, Pleistophora, Trachipleistophora, Vittaforma, Microsporidium, Nosema, Encephalitozoon, and Enterocytozoon.

Practical Guide to Diagnostic Parasitology

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