Читать книгу Practical Guide to Diagnostic Parasitology - Lynne Shore Garcia - Страница 89

1. Adequate fixation still depends on the following factors:

a. Meeting recommended time limits for lag time between passage of the specimen and fixation

b. Use of the correct ratio of fixative to specimen (3:1)

c. Thorough mixing of the fixative and specimen (once the specimen is received in the laboratory, any additional mixing will not counteract the lack of fixative-specimen mixing and contact prior to that time)

2. Unless the appropriate stain is used with each fixative, the final permanent stained smear may be difficult to examine (organisms may be hard to see and/or identify). Examples of appropriate combinations are

a. Schaudinn’s or PVA fixative with trichrome or iron hematoxylin stains

b. SAF fixative with iron hematoxylin stain

c. Single-vial mercuric chloride substitute systems with trichrome, iron hematoxylin, or company-developed proprietary stains matched to their specific fixatives