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1 A PRIMERFigure 1aFigure 1bFigure 2Figure 3Figure 4Figure 5Figure 6Figure 7Figure 8 PCR. (A) In the first cycle, a double-stranded DNA target sequence is u...Figure 9 bDNA-based signal amplification. Target nucleic acid is released by dis...Figure 10Figure 11 Left disk, optochin; right disk, oxacillin.Figure 12Figure 13Figure 14

2 CASE 1Figure 1.1Figure 1.2

3 CASE 2Figure 2.1

4 CASE 5Figure 5.1

5 CASE 7Figure 7.1

6 CASE 8Figure 8.1Figure 8.2

7 CASE 9Figure 9.1 Organism infecting this patient.

8 CASE 10Figure 10.1 Amplification curves of a real-time PCR test.

9 CASE 12Figure 12.1 Colonies on buffered charcoal yeast extract (BCYE) agar (left) and 5...Figure 12.2 Gram stain of colonies seen in Fig. 12.1.

10 CASE 13Figure 13.1Figure 13.2Figure 13.3

11 CASE 14Figure 14.1 Calcofluor white stain of biopsy material from patient.Figure 14.2 Organism growing from patient’s transbronchial biopsy.

12 CASE 15Figure 15.1 Calcofluor white examination of left ethmoid sinus biopsy.Figure 15.2 Culture from sinus specimen.

13 CASE 16Figure 16.1Figure 16.2

14 CASE 17Figure 17.1Figure 17.2Figure 17.3Figure 17.4Figure 17.5 C. immitis spherules (from reference 5 with permission).

15 CASE 18Figure 18.1 Papanicolaou staining of gastric brushing.

16 CASE 19Figure 19.1

17 CASE 20Figure 20.1 Gram stain of the organism that was recovered from a positive blood ...Figure 20.2 A subculture of the organism from the positive blood culture demonst...Figure 20.3 Susceptibility test for the organism that was isolated from the pati...

18 CASE 21Figure 21.1Figure 21.2

19 CASE 22Figure 22.1Figure 22.2

20 CASE 24Figure 24.1

21 CASE 26Figure 26.1 Immunochromatographic test for GDH and toxins A and B from C. diffic...Figure 26.2 The pseudomembranous lesions are the yellow-green areas on the surfa...

22 CASE 27Figure 27.1 Material extracted from anus.Figure 27.2 Material obtained from crushing segment of material in Fig. 27.1.

23 CASE 28Figure 28.1

24 CASE 29Figure 29.1 Organisms recovered from blood on Hektoen agar (black colonies indic...Figure 29.2 TSI slant of organism recovered from blood (courtesy L. M. de la Maz...Figure 29.3 Pulsed-field gel electrophoresis fingerprints of Salmonella (from U....

25 CASE 30Figure 30.1

26 CASE 31Figure 31.1 Gram stain of organism growing from blood culture bottle.Figure 31.2 Organism growth on MacConkey agar after 24 hours’ incubation at 35°C...

27 CASE 32Figure 32.1 Serologic and clinical patterns observed during acute HBV infection....Figure 32.2 Typical sequence of serologic markers in patients with acute hepatit...

28 CASE 34Figure 34.1 Testing algorithm for the diagnosis of hepatitis C infection. aIf ac...

29 CASE 35Figure 35.1Figure 35.2 The tube on the left (yellow color) is a negative control; the tube ...

30 CASE 36Figure 36.1Figure 36.2Figure 36.3Figure 36.4 Disk diffusion susceptibility test of patient’s isolate.

31 CASE 37Figure 37.1Figure 37.2

32 CASE 38Figure 38.1Figure 38.2

33 CASE 39Figure 39.1 Direct Gram stain from tissue.Figure 39.2 Isolate recovered from patient.Figure 39.3 D test with erythromycin (E) and clindamycin (CC).

34 CASE 40Figure 40.1 Kinyoun stain of biopsy of right hand (×1,000 magnification).Figure 40.2 Organism prior to (left) and after (right) light exposure.

35 CASE 41Figure 41.1

36 CASE 42Figure 42.1

37 CASE 43Figure 43.1 Initial lesion in Peru.Figure 43.2 Lesion 1 month later at time of punch biopsy.Figure 43.3 Microscopic examination from skin biopsy (magnification, ×1,000).Figure 43.4 Organism grown on culture from the biopsy (magnification, ×400).

38 CASE 44Figure 44.1 CSF Gram stain from patient.Figure 44.2 E-test susceptibility test of the organism grown from the patient’s ...Figure 44.3 Optochin disks showing zone of growth inhibition characteristic of S...

39 CASE 45Figure 45.1 Gram stain of the organism isolated from the placental culture.Figure 45.2 Organism growing on 5% sheep blood agar. Note that the organism is w...

40 CASE 46Figure 46.1Figure 46.2

41 CASE 48Figure 48.1Figure 48.2Figure 48.3Figure 48.4 Patient isolate on right; negative control on left.

42 CASE 50Figure 50.1Figure 50.2

43 CASE 51Figure 51.1Figure 51.2

44 CASE 53Figure 53.1Figure 53.2Figure 53.3 S. lugdunensis on sheep blood agar.

45 CASE 54Figure 54.1Figure 54.2

46 CASE 55Figure 55.1 Patient’s peripheral blood smear.Figure 55.2 TSI slant of isolate recovered from blood culture.

47 CASE 56Figure 56.1 Gram stain from blood culture bottle.Figure 56.2 Organism growing on chocolate agar.Figure 56.3 PNA FISH of blood culture.

48 CASE 57Figure 57.1Figure 57.2

49 CASE 58Figure 58.1Figure 58.2Figure 58.3

50 CASE 59Figure 59.1 Patient’s rash on the back and upper arm.Figure 59.2 Patient’s rash on palms.Figure 59.3 Syphilis testing algorithms.

51 CASE 61Figure 61.1

52 CASE 62Figure 62.1 Parvovirus B19 infection in a healthy individual. (From Brown KE, Yo...

53 CASE 63Figure 63.1

54 CASE 64Figure 64.1 Gram stain of positive blood culture.Figure 64.2 N. meningitidis meningitis belt in central sub-Saharan Africa (from ...Figure 64.3 Large lesions are called purpura; small pinpoint lesions are called ...

55 CASE 65Figure 65.1

56 CASE 66Figure 66.1 AP portable chest X ray.Figure 66.2 Hantavirus pulmonary syndrome cases reported by state, United States...

57 CASE 67Figure 67.1 Sinus biopsy.Figure 67.2 Skin lesion at time of positive blood culture.Figure 67.3 Positive blood culture.Figure 67.4 Macroconidia of organism infecting this patient.

58 CASE 68Figure 68.1Figure 68.2Figure 68.3

59 CASE 69Figure 69.1 Portable chest X ray of patient following transfer.Figure 69.2 Growth on chocolate agar. (Courtesy Luis de la Maza.)Figure 69.3 Reported tularemia cases, United States, 2003 to 2012. (http://www.c...

60 CASE 70Figure 70.1 Chest computed tomography scan showing diffuse, tiny nodular lesions...Figure 70.2 Stain of bronchoalveolar lavage fluid.

61 CASE 71Figure 71.1 Ocular lesion 7 days posttrauma.Figure 71.2 Calcofluor white examination of corneal scraping.Figure 71.3 Organism recovered from corneal scraping at 7 days of incubation at ...Figure 71.4 Lactophenol cotton blue microscopic view of the organism in Fig. 71....

62 CASE 72Figure 72.1 Organism growing on MacConkey agar.

63 CASE 73Figure 73.1 Wet-mount examination of aspirate of cystic mass enhanced by methyle...Figure 73.2 Hematoxylin and eosin stain of tissue removed from intra-abdominal l...

64 CASE 74Figure 74.1 Patient’s left leg prior to amputation.Figure 74.2 Gram stain from a biopsy from the leg of the patient.

Cases in Medical Microbiology and Infectious Diseases

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