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11

On the Definition of Prokaryotes

by Nanne Nanninga

As will be argued below, the present definition of a prokaryote is highly unsatisfactory. To give an example: a prokaryote is “a cell or organism lacking a nucleus and other membrane-enclosed organelles, usually having its DNA in a single circular molecule” (Brock, Biology of Microorganisms, 10^th ed.). This seems a summary of the original definition of Stanier & van Niel (1962), which I quote for the sake of completeness: “The principle distinguishing features of the procaryotic cell are: 1. absence of internal membranes which separate the resting nucleus from the cytoplasm, and isolate the enzymatic machinery of photosynthesis and of respiration in specific organelles; 2. nuclear division by fission, not by mitosis, a character possibly related to the presence of a single structure which carries all the genetic information of the cell; and 3. the presence of a cell wall which contains a specific mucopeptide as its strengthening element.” Today’s perception of these points amounts largely, as indicated above, to the absence of a nuclear envelope in prokaryotes. It should be mentioned that Stanier & van Niel in the above paper also wished to differentiate a bacterium from a virus and to incorporate blue-green algae within the prokaryotic domain.

The paper of Stanier & van Niel appeared more than 50 years ago. In this contribution I will attempt to present a more modern definition of a prokaryote, keeping in mind that one should distinguish between two aspects: its definition and its distinction from a eukaryote. But, before doing so I will insert an historic intermezzo.

Intermezzo

An alternative characterization of prokaryotes already emerged, though not explicitly, in 1964 through the study by Byrne et al. of the formation of DNA-ribosome complexes as analyzed by sucrose-gradient centrifugation. In those times sucrose gradient data were graphically expressed with radioactive counts on the vertical axis and fraction number on the horizontal axis. In the paper of Byrne et al. the focus was on the cellular organization of transcription and on the stability of RNAs, in particular mRNA (then also named cRNA). Their labeling and fractionation experiments led to the following conclusion: “Our results suggest that some time after the onset of cRNA transcription on the surface of the gene, a ribosome will attach to the RNA molecule. This event may signal the beginning of polypeptide synthesis on the ribosome. The ribosome may then advance along the cRNA strand followed by other ribosomes, resulting in a continuous translation of the genetic message into polypeptide sequences.” And further on: “In a sense, ribosomes in bacterial systems may be a rudimentary form of the “carrier” ribonucleoprotein particles believed to transport cRNA from the nuclei of higher organisms.” An electron micrograph of one of the fractions shows clusters of particles with ribosomal dimensions and threads, presumed to be DNA and perhaps mRNA.

Dispersed strands of genomic DNA with polysomes attached of the hyper-thermophilic archaeon Thermococcus kodakaraensis visualized by the Miller technique (1970). Bar: 200 nm.

Source: French SL, Santangelo TJ, Beyer AL, Reeve JN. 2007. Transcription and translation are coupled in Archaea. Molecular Biology and Evolution 24:893-895.

Protection of nascent transcribed RNA was also a concern of G. S. Stent (1966): “What kind of system might assure in vivo removal of the nascent RNA from the template?” In the case of mRNA, ribosomes came to the fore and mRNA-protection became incorporated into the framework of coupled transcription and translation; that is, translation starts before transcription has been finished. The conceptual drawing of Stent became reality through the impressive electron micrographs of Miller et al. (1970).

Then, E. coli was still the model organism. Some years ago, coupled transcription and translation was visualized for the hyperthermophilic archaeon Thermococcus kodakaraensis using the Miller technique (French et al. 2007). Thus, one can conclude that in the bacterium E. coli and in the archaeon T. kodakaraensi coupled transcription and translation are facts. Whether this applies to all Archaea remains to be seen. Because eukaryotes lack coupled transcription and translation, prokaryotes, in this way, distinguish themselves positively (Martin & Koonin, 2006).

The Distinction between Prokaryotes and Eukaryotes

As mentioned above, one should differentiate between definition and distinction. The fact that eukaryotes do not possess coupled transcription and translation—a negative qualification for some, by the way—does not tell us much about eukaryotes. Also, the absence of a nuclear envelope in prokaryotes—a negative qualification, too—is not very informative. What is fundamental in cells relates to the structural organization of information processing, i.e., the flow of information from DNA through mRNA to protein. Conceptually, this bears on the central dogma of molecular biology. Thus, the main distinction between pro- and eukaryotes lies in the presence or absence of coupled transcription and translation, respectively. Does this suffice as a definition of a prokaryote? This question is easier posed than answered. I refer to another quotation from Stanier & van Niel in the same paper mentioned above: “The differences between eucaryotic and procaryotic cells are not expressed in any gross features of cellular function; they reside rather in differences with respect to the detailed organization of the cellular machinery” (italics by Stanier & van Niel). The absence of a cellular structure (a nuclear envelope) can hardly be considered an informative statement when it is known that transcription and translation are spatially coupled. The latter reflects the “detailed organization of the cellular machinery” and, I believe, this should be part of a definition, as should microscopic size and a cell wall. If one contrasts prokaryotic cytoplasm with that of a eukaryote, the presence of membrane-bounded organelles suspended in a dynamic cytoskeletal framework appears a dominant feature in the latter case. Such structural differentiation could hardly be accommodated for in a microscopic cell. Also remember that mitochondria and chloroplasts have prokaryotic dimensions. Following this reasoning, a prokaryote can be considered a walled cell of microscopic size possessing coupled transcription and translation and a not-well differentiated cytoplasm. According to our present knowledge, this applies to Bacteria and Archaea. Structurally, there is a “deep gulf” (Lane, 2011) between prokaryotes and eukaryotes. To what extent this bears on their phylogenetic relationships remains to be seen.

Structural organization of the flow of information in pro- and eukaryotes.

Credit: Nanne Nanninge.

Two observations seem at variance with the foregoing. First, there is the remarkable membrane compartmentation of a planctomycete bacterium like Gemmata obscuriglobus. However, three-dimensional reconstructions suggest that the visual compartments are due to a sectioned lobed conformation of an E. coli-reminiscent organism (Santarella-Mellwig et al. 2013), implicating a prokaryotic organization. Second, nuclei do not seem to be devoid of protein synthesis, though the nature of the proteins involved are not known (David et al. 2012). Presumably, an exception that might prove the rule.

Nanne Nanninga is Emeritus Professor of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands.

References

Madigan MT, Martinko JM, Parker J. 2003. Brock Biology of Microorganisms. 10th edition. Pearson Education, Inc., Upper Saddle River, NJ, USA.

Byrne R, Levin JG, Bladen HA, Nirenberg MW. 1964. The in vitro formation of a DNA-ribosome complex. Proc Natl Acad Sci USA 52:140–148.

David A, Dolan BP, Hickman HD, Knowlton JJ, Clavarino G, Pierre P, Bennink JR, Yewdell JW. 2012. Nuclear translation visualized by ribosome-bound nascent chain puromycylation. J Cell Biol 197:45–57.

French SL, Santangelo TJ, Beyer AL, Reeve JN. 2007. Transcription and translation are coupled in Archaea. Mol Biol Evol 24:893–895.

Lane N. 2011. Energetics and genetics across the prokaryote-eukaryote divide. Biol Direct 6:35.

Martin W, Koonin EV. 2006. A positive definition of prokaryotes. Nature 442:868.

Miller OL Jr, Hamkalo BA, Thomas CA Jr. 1970. Visualization of bacterial genes in action. Science 169:392–395.

Santarella-Mellwig R, Pruggnaller S, Roos N, Mattaj IW, Devos DP. 2013. Three-dimensional reconstruction of bacteria with a complex endomembrane system. PLoS Biol 11:e1001565.

Stanier RY, Van Niel CB. 1962. The concept of a bacterium. Arch Mikrobiol 42:17–35.

Stent GS. 1966. Genetic transcription. Proc R Soc Lond B Biol Sci 164:181–197.

November 2, 2014

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