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Used in the preparation of bone, tooth, osseous tumours, &c. The two best fluids for general use are:—

Chromic and nitric fluid.—This is made as follows:—

Chromic acid	1	gramme	45	grains.
Nitric acid	2	grammes	1 1/2	drachms.
Water	200	c.c.	1	pint.

If the bone is not very compact the fluid may be used diluted with an equal quantity of water. A large quantity of fluid should be used, and like all decalcifying fluids, it should be frequently changed.

As soon as the specimen is sufficiently flexible, it should be thoroughly washed in running water for some hours, and then transferred to spirit until it is convenient to cut sections.

Von Ebner’s solution:—

Hydrochloric acid	1	gramme	1 1/2	drachms.
Common salt	10	grammes	2	ounces.
Water to	100	c.c.	1	pint.

It is a very useful decalcifying agent, but causes the fibrous elements to swell up rather more than chromic and nitric fluid. A large quantity of the fluid must be used, and it should be changed daily. It must be very thoroughly washed out in running water when the decalcification is completed.

Bleaching solution (eau de Javelle).