Читать книгу Structure and Function of the Bacterial Genome - Charles J. Dorman - Страница 2

1  Cover

2  Dedication

3  Preface

4  1 The Bacterial Genome – Where the Genes Are 1.1 Genome Philosophy 1.2 The Bacterial Chromosome 1.3 Chromosome Replication: Initiation 1.4 Chromosome Replication: Elongation 1.5 Chromosome Replication: Termination 1.6 Replication Produces Physically Connected Products 1.7 Decatenating the Sister Chromosomes 1.8 Resolving Chromosome Dimers 1.9 Segregating the Products of Chromosome Replication 1.10 Polar Tethering of Chromosome Origins 1.11 Some Bacterial Chromosomes Are Linear 1.12 Some Bacteria Have More than One Chromosome 1.13 Plasmids 1.14 Plasmid Replication 1.15 Plasmid Segregation 1.16 The Nucleoid 1.17 The Chromosome Has Looped Domains 1.18 The Macrodomain Structure of the Chromosome 1.19 The Chromosome Displays Spatial Arrangement Within the Cell 1.20 SeqA and Nucleoid Organisation 1.21 MukB, a Condensin‐Like Protein 1.22 MatP, the matS Site and Ter Organisation 1.23 MaoP and the maoS Site 1.24 SlmA and Nucleoid Occlusion 1.25 The Min System and Z Ring Localisation 1.26 DNA in the Bacterial Nucleoid 1.27 DNA Topology 1.28 DNA Topoisomerases: DNA Gyrase 1.29 DNA Topoisomerases: DNA Topoisomerase IV 1.30 DNA Topoisomerases: DNA Topoisomerase I 1.31 DNA Topoisomerases: DNA Topoisomerase III 1.32 DNA Replication and Transcription Alter Local DNA Topology 1.33 Transcription and Nucleoid Structure 1.34 Nucleoid‐associated Proteins (NAPs) and Nucleoid Structure 1.35 DNA Bending Protein Integration Host Factor (IHF) 1.36 HU, a NAP with General DNA‐binding Activity 1.37 The Very Versatile FIS Protein 1.38 FIS and the Early Exponential Phase of Growth 1.39 FIS and the Stringent Response 1.40 FIS and DNA Topology 1.41 Ferritin‐Like Dps and the Curved‐DNA‐binding Protein CbpA 1.42 The H‐NS Protein: A Silencer of Transcription 1.43 StpA: A Paralogue of H‐NS 1.44 H‐NS Orthologues Encoded by Plasmids and Phage 1.45 H‐NSB/Hfp and H‐NS2: H‐NS Homologues of HGT Origin 1.46 A Truncated H‐NS‐Like Protein 1.47 Hha‐like Proteins 1.48 Other H‐NS Homologues: The Ler Protein from EPEC 1.49 H‐NS Functional Homologues 1.50 H‐NS Functional Homologues: Rok from Bacillus spp. 1.51 H‐NS Functional Homologues: Lsr2 from Actinomycetes 1.52 H‐NS Functional Homologues: MvaT from Pseudomonas spp. 1.53 The Leucine‐responsive Regulatory Protein, LRP 1.54 Small, Acid‐soluble Spore Proteins, SASPs

5  2 Conservation and Evolution of the Dynamic Genome 2.1 Disruptive Influences: Mutations 2.2 Repetitive Sequences in the Chromosome and Their Influence on Genetic Stability 2.3 Contingency Loci and the Generation of Microbial Variety 2.4 Rhs: Rearrangement Hotspots 2.5 REP Sequences 2.6 RIB/RIP, BIME‐1, and BIME‐2 Elements 2.7 ERIC Sequences 2.8 Repeat‐Mediated Rearrangements: Mechanisms and Frequency 2.9 Site‐specific Recombination and Phenotypic Variety 2.10 Site‐Specific Recombination: Bacteriophage Lambda 2.11 The Lambda Lysis/Lysogeny Decision 2.12 Tyrosine Integrases 2.13 Serine Invertases 2.14 Large Serine Recombinases 2.15 Transposition and Transposable Elements 2.16 Pathways of Transposition 2.17 Peel‐and‐paste Transposition 2.18 Control of Transposition 2.19 Host Factors and Transposition 2.20 Integrative and Conjugative Elements (ICE) 2.21 Integrons 2.22 Introns 2.23 Horizontal Gene Transfer 2.24 Distinguishing Self from Non‐self 2.25 Distinguishing Self and Non‐self: CRISPR‐Cas Systems 2.26 Distinguishing Self and Non‐self: Argonaute Proteins 2.27 Distinguishing Self and Non‐self: Restriction Enzymes/Methylases 2.28 Distinguishing Self and Non‐self: BREX 2.29 Self‐sacrifice and Other Behaviours Involving Toxin—antitoxin Systems 2.30 Conservative Forces: DNA Repair and Homologous Recombination 2.31 The RecA Protein 2.32 RecA, LexA, and the SOS Response 2.33 Holliday Junction Resolution 2.34 Mismatch Repair 2.35 Non‐homologous End Joining

6  3 Gene Control: Transcription and Its Regulation 3.1 Transcription: More Than Just Transcribing Genetic Information 3.2 RNA Polymerase 3.3 The Core Enzyme 3.4 The Sigma Factors (and Anti‐Sigma Factors) 3.5 Promoter Architecture 3.6 Stringently Regulated Promoters 3.7 Transcription Factors and RNA Polymerase 3.8 Transcription Initiation 3.9 Transcription Elongation 3.10 Transcription Termination: Intrinsic and Rho‐Dependent Terminators 3.11 Rho and Imported Genes 3.12 Rho, R‐Loops, and DNA Supercoiling 3.13 Rho and Antisense Transcripts 3.14 Anti‐Termination: Insights from Phage Studies 3.15 Transcription Occurs in Bursts

7  4 Gene Control: Regulation at the RNA Level 4.1 Antisense Transcripts and Gene Regulation incis 4.2 RNA that Regulates intrans 4.3 DsrA and the RpoS/H‐NS Link 4.4 sRNA Turnover 4.5 DEAD‐box Proteins 4.6 RNA Chaperone Proteins 4.7 StpA, H‐NS, and RNA Binding 4.8 Degradation of mRNA 4.9 RNA Folding and Gene Regulation 4.10 Transcription Attenuation 4.11 Riboswitches 4.12 RNA as a Structural Component in the Nucleoid

8  5 Gene Control: Regulation at the Protein Level 5.1 Control Beyond DNA and RNA 5.2 Translation Machinery and Control: tRNA and rRNA 5.3 Translation Machinery and Control: The Ribosome 5.4 Translation Initiation 5.5 Translation Elongation 5.6 Elongation Factor P (EF‐P) 5.7 Translation Termination 5.8 Protein Secretion 5.9 Protein Secretion: The Sec Pathway 5.10 The Twin Arginine Translocation (Tat) Pathway of Protein Secretion 5.11 Type 1 Secretion Systems (T1SS) 5.12 Type 2 Secretion Systems (T2SS) 5.13 Type 3 Secretion Systems (T3SS) 5.14 Type 4 Secretion Systems (T4SS) 5.15 Type 5 Secretion Systems (T5SS): The Autotransporters 5.16 Type 6 Secretion Systems (T6SS) 5.17 Protein Secretion in Gram‐Positive Bacteria: SecA1, SecA2, and SrtA 5.18 Type 7 Secretion Systems (T7SS) 5.19 Protein Modification: Acetylation 5.20 Protein Modification: Glycosylation 5.21 Protein Modification: Phosphorylation 5.22 Protein Splicing 5.23 Small Proteins 5.24 Selenocysteine and Pyrrolysine: The 21st and 22nd Amino Acids

9  6 Gene Control and Bacterial Physiology 6.1 The Bacterial Growth Cycle 6.2 Physiology Changes Throughout the Growth Cycle 6.3 Generating Physiological Variety from Genetic Homogeneity 6.4 Bacterial Economics – Some Basic Principles 6.5 Carbon Sources and Metabolism 6.6 Gene Control and Carbon Source Utilisation 6.7 Anaerobic Respiration 6.8 ArcA, Mobile Genetic Elements, and HGT 6.9 Stress and Stress Survival in Bacterial Life 6.10 Oxygen Stress 6.11 Iron Starvation 6.12 Siderophores and Iron Capture 6.13 TonB‐Dependent Transporters 6.14 Gene Regulation and Iron Transport 6.15 Iron Storage and Homeostasis 6.16 Osmotic Stress and Water Relations in Bacteria 6.17 Signal Molecules and Stress 6.18 The Stringent Response 6.19 Regulation of the Acid Stress Response 6.20 Alkaline pH Stress Response 6.21 Motility and Chemotaxis 6.22 Quorum Sensing 6.23 Biofilms 6.24 ‘Cheating’ as a Lifestyle Strategy 6.25 Thermal Regulation 6.26 Epigenomics and Phasevarions 6.27 Some Unifying Themes

10  7 Gene Control: Global Regulation by H‐NS 7.1 H‐NS Is a Global Regulator 7.2 H‐NS and Foreign DNA 7.3 H‐NS and Xenogenic Silencing: Three Case Studies 7.4 The H‐NS Virulence Regulon in Vibrio cholerae 7.5 HGT in V. cholerae: The CTXφ Phage and the VPI1 Island 7.6 The ToxRS, ToxT, TcpPH Regulatory Network 7.7 Control by VpsR, VpsT, and HapR 7.8 Quorum Sensing and Cholera 7.9 Chitin and HGT 7.10 The H‐NS Virulence Regulon in Shigella flexneri 7.11 Shigella Infection 7.12 The VirF AraC‐Like Transcription Factor 7.13 VirB: A Recruit from a Plasmid‐Partitioning System 7.14 The Shigella Virulence Plasmid 7.15 The Salmonella H‐NS Virulence Gene Regulon 7.16 Salmonella's Pathogenicity Islands (SPI) 7.17 SlyA, PhoP/Q, and SPI Gene Expression 7.18 Gene Control in SPI1 and SPI2

11  8 An Integrated View of Genome Structure and Function 8.1 Networks versus Hierarchies 8.2 Regulons, Stimulons, and Heterarchies/Netarchies 8.3 Transcription Burstiness and Regulatory Noise 8.4 The Significance of Gene Position 8.5 Messenger RNA May Not Be Free to Diffuse Far in Bacteria 8.6 RNA Polymerase Activity and Genome Organisation 8.7 Gene–Gene Interactions in the Folded Chromosome 8.8 DNA Supercoiling as a Global Regulator 8.9 Modelling the Nucleoid 8.10 Synthetic Biology

12  References

13  Index

14  End User License Agreement