Читать книгу Principles of Plant Genetics and Breeding - George Acquaah - Страница 329

The subject is discussed in detail in Chapter 22. Inducing variability via mutagenesis is challenging for two key reasons. Being rare events, a large population of M₁V₂ is needed to have a good chance of observing desired mutants. Obtaining a large number of vegetative propagules is difficult. Also, mutations occur in individual cells. Without the benefit of meiosis, the mutated clonal material develops chimeras. Using adventitious buds as starting material reduces the chance of chimeras. A mutation in the epidermal cell (usually there is one) would result in an adventitious shoot that originated from a single mutant cell. This technique is not universally applicable.