Читать книгу Principles of Plant Genetics and Breeding - George Acquaah - Страница 370
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ОглавлениеInbred lines are homozygous genotypes produced by repeated selfing with selection over several generations. The technique of doubled haploids may be used to produce complete homozygous diploid lines in just one year (versus more than four years in conventional breeding) by doubling the chromosome complement of haploid cells. Such doubling may be accomplished in vivo naturally or through crossing of appropriate parents, or in vitro, through the use of colchicine. The success of doubled haploids as a breeding technique depends on the availability of a reliable and efficient system for generating haploids and doubling them in the species.