Читать книгу EXTREMOPHILES as Astrobiological Models - Группа авторов - Страница 24

To control for the presence of foreign DNA in our sample analysis of DNA from steam-condensed samples, we used Millipore filtered (0.22 µm membrane) autoclaved Barnstead purified water. The water was converted to steam next to a cave or vent sampled and collected in identical fashion to steam samples from fumaroles. After collection, samples were analyzed with cave or vent samples by (1) PCR, (2) filtration of 10–15 mL samples on 13 mm black Millipore membranes (0.22 µm pore size) and DAPI microscopy, and (3) for thermophile culture pH 3 and 4.5 at 55, 70, 80, and 85 °C. Control samples proved to be negative.