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A curvilinear (microconvex) probe is most frequently used to image the spleen, and the MHz is adjusted for the size of the animal. Smaller dogs and cats are generally imaged using higher frequencies of 7.5–10 MHz (less penetration, better detail) and large dogs are generally imaged using lower frequencies such as 5 MHz (deeper penetration, less detail). In small dogs and cats, a linear probe may also be used in order to maximize image quality (due to the higher resolution) when greater penetration depth is not of concern.

Patients are most commonly imaged in dorsal recumbency. In large dogs, however, the cranial aspect of the spleen is often located under the ribcage on the left side. Repositioning these patients into right lateral recumbency allows visualization of the cranial aspect or head of the spleen using a left intercostal approach (Hecht 2008). The authors recommend performing a Global FAST to screen for any intrathoracic problems including pleural and pericardial effusion, cardiac and pulmonary conditions, and abnormal volume status that may pose patient risk before moving the patient into dorsal recumbency.