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The morphology of the wax-oil systems was studied using a HITACHI Ultra-High Resolution Scanning Electron Microscope model S-4800 at an accelerating voltage of 5 kV.

For SEM sample preparation, the procedure for extracting oils out of the wax was followed as reported by Yoshida and coworkers [16] or by Miyazaki and Marangoni [19]. In our study, a part of lipstick was used and its oils were extracted using ethanol, acetone and tetrahydrofuran (THF). It was observed that THF was the best solvent for extracting the oils without damaging the samples for SEM experiment. The lipstick sample preparation is shown in Figure 2.4.