Читать книгу Gastrointestinal Pathology - Группа авторов - Страница 19

Cytology samples obtained from either brush or fine‐needle aspiration can be processed in a variety of ways. Most commonly these are prepared as thin smear on glass slides, which can be evaluated either immediately as an air‐dried sample stained with a modified Romanowsky stain, or as an alcohol‐fixed slide evaluated with Papanicolaou staining. It is often helpful to place additional excess material in a cytological preservative or formalin. Special handling is required for samples where lymphoproliferative disease is considered. Typically these include cytological preservatives such as Roswell Park Memorial Institute (RPMI) medium that allow subsequent flow cytometry. As a practical matter, placement in such a preservative should be considered even when likelihood is low since cells preserved in such solution can always be examined with routine cytological methods; however, cells that are placed in formalin or alcohol cannot be evaluated for flow cytometry. The use of rapid on‐site evaluation (ROSE) cytology has been shown in many studies to increase diagnostic yield and reduce the need for repeated procedures.

Figure 1.10 Pinned and oriented resection tissue from Barrett's esophagus‐associated neoplasia. Note the mucosal side facing up and the lateral margins pinned to prevent curling of the edges.