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In the Netherlands, BBSE procedures closely follow those described by the SFT [21]. The reason for mentioning the BBSE procedures used in this region is to highlight the emphasis placed on consistently diluting semen in order to gain an accurate individual motility assessment. Bulls can fail a BBSE based on a poor individual motility assessment, and poor semen handling or an inability to clearly visualize individual sperm can adversely affect the result. In the Netherlands, a routine 1 : 80 dilution of semen with physiological saline warmed to 37 °C prior to individual motility assessment is described. In the absence of direct concentration estimates, such a process will result in diluted concentrations of 2.5 × 10⁶ to 13 × 10⁶ cells/ml in the assessment sample. Concentrations such as this allow clear visualization of individual spermatozoa. This process is relatively easily performed in the field by adding 790 μl of warmed saline or PBS to 10 μl of semen.