Читать книгу Bovine Reproduction - Группа авторов - Страница 115

In all systems except that of the ACV, the use of nigrosin‐eosin staining is one of the recommendations for preparing samples for morphological examination. None of the regions recommends incorporating the Live/Dead aspect of vital staining into the BBSE assessment. The ACV does not recommend the use of vital stains for morphology assessment due to the recommendation of utilizing an endorsed morphologist for the assessment process. In this scenario, the morphologists unanimously prefer samples in fixative such as formal buffered saline (FBS) solution as this enhances the detection of defects under phase‐contrast or DIC microscopy. There is also concern that the osmolality of the vital stain may vary widely if not stored carefully, adversely affecting sperm morphology.