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The authors would like to gratefully acknowledge Drs. Kevin T. Bush, Megha Nagle, David M. Truong, Vibha Bhatnagar, Gregory Kaler, Satish A. Eraly, and Wei Wu for their contributions to previous editions of this chapter. We also wish to thank other members of the Nigam lab who have, over many years, contributed to the material discussed here. Finally, we would like to thank Zhengxuan Liang, from Dr. You’s lab, for her help during the revision process.