Читать книгу Principles of Virology, Volume 1 - Jane Flint, S. Jane Flint - Страница 102

The idea that one-step growth analysis can be used to study the single-cell reproductive cycle of viruses originated from the work on bacteriophages by Emory Ellis and Delbrück. In their classic experiment, they added virus particles to a culture of rapidly growing E. coli. These particles adsorbed quickly to the cells. The infected culture was then diluted, preventing further adsorption of unbound particles. This simple dilution step is the key to the experiment: it reduces further binding of virus to cells and effectively synchronizes the infection. Samples of the diluted culture were then taken every few minutes and analyzed for the number of infectious bacteriophages.

When the results of this experiment were plotted, several key observations emerged. The graphs were surprising in that they did not resemble the growth curves of bacteria or cultured cells. After a short lag, bacterial cell growth becomes exponential (i.e., each progeny cell is capable of dividing into two cells) and follows a straight line (Fig. 2.18A). Exponential growth continues until the nutrients in the medium are exhausted. In contrast, numbers of new viruses do not increase in a linear fashion from the start of the infection (Fig. 2.18B, left). There is an initial lag period in which no infectious viruses can be detected. This lag period is followed by a rapid increase in the number of infectious particles, which then plateaus. The single cycle of virus reproduction produces this “burst” of virus progeny. If the experiment is repeated, such that only a few cells are initially infected, the graph looks different (Fig. 2.18B, right). Instead of a single cycle, there is a stepwise increase in numbers of new viruses with time. Each step represents one cycle of virus infection.

Figure 2.17 Workflow for VS-Virome. Shown is the computational pipeline designed for defining the type and abundance of known and novel viral sequences in metagenomic data sets. VS-Virome first pre-processes the sequences (left) to remove adapter sequences (these are added to every DNA in the sample, and contain barcoding sequences, primer binding sites, and sequences for immobilizing the DNA), joins paired end reads if they overlap, performs quality control on sequences, and identifies low-complexity sequences and host sequences before subjecting all the sequences to BLAST (right) to detect viral sequences. Because integrated prophage are found in bacterial genomes, alignment to comprehensive databases could lead to removal of bona fide bacteriophage sequences. Bacteriophage hits are therefore placed into a separate output file. Candidate eukaryotic viral sequences are filtered to remove sequences that have high identity to bacterial genomes. Remaining reads are then aligned to the more comprehensive GenBank NT and NR databases to identify reads or contigs that have greater similarity to nonviral sequences than to viral sequences (i.e., increased likelihood of being a false positive). To have a high degree of confidence in viral classification, sequences that have significant hits to both viral and any nonviral reference sequence are placed in an “ambiguous” bin. Sequences in the viral bin only have significant alignment to viral sequences.

Once the nature of the viral propagation cycle was explored using the one-step growth curve, questions emerged about what was happening in the cell before the burst. What was the fate of the incoming virus? Did it disappear? How were more virus particles produced? These questions were answered by looking inside the infected cell. Instead of sampling the diluted culture for virus after various periods of infection, researchers prematurely lysed the infected cells as the infection proceeded and then assayed for infectious virus. The results were extremely informative. Immediately after dilution, there was a complete loss, or eclipse, of infectious virus for 10 to 15 min (Fig. 2.18B). In other words, input virions disappeared, and no new phage particles were produced during this period. It was shown later that the loss of infectivity is a consequence of the release of the genome from the virion, to allow for subsequent transcription of viral genes. Particle infectivity is lost during this phase because the released genome is not infectious under the conditions of the plaque assay. Later, newly assembled infectious particles could be detected inside the cell that had not yet been released by cell lysis.