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Embryonated Eggs

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Before the advent of cell culture, many viruses were propagated in embryonated chicken eggs (Fig. 2.6). At 5 to 14 days after fertilization, a hole is drilled in the shell and virus is injected into the site appropriate for its replication. This method of virus propagation is now routine only for influenza virus. The robust yield of this virus from chicken eggs has led to their widespread use in research laboratories and for vaccine production.

Principles of Virology, Volume 1

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