Читать книгу Chemistry and Biology of Non-canonical Nucleic Acids - Naoki Sugimoto - Страница 55

1 Study the interactions to determine the stability of canonical nucleic acids.

Stability of canonical nucleic acids depends on the sequences because hydrogen bonding, base stacking, and conformational entropy affect mainly stability of canonical nucleic acids.

1 Understand the difference in factors determining stability of canonical and non-canonical nucleic acids.

Factors influencing the non-canonical nucleic acids are different from that of canonical duplex. The non-canonical structures are susceptible to a change in solution condition such as pH and cations. The difference can appear as structural properties, especially charge of the nucleic acids and protonation of bases.

1 Analyze the stability for canonical and non-canonical nucleic acids.

The thermodynamic parameters for both canonical and non-canonical structures can be estimated using thermal melting curves for the nucleic acid structures. The different equations for melting treatments are required because the unfolding process for the nucleic acids depends on their structures.