Читать книгу Histology of the Blood, Normal and Pathological - Paul Ehrlich - Страница 11

A simple plate of copper on a stand is used, under one end of which burns a Bunsen flame. After some time a certain constancy in the temperature of the plate is reached, the part nearest to the flame is hottest, that farther away is cooler. By dropping water, toluol, xylol, etc. on to it, one can fairly easily ascertain that point of the plate which has reached the boiling temperature of the particular fluid.

Far more convenient is Victor Meyer's apparatus, used by chemists. This consists of a copper boiler, modified for our purpose, with a roof of thin copper-plate, perforated for the opening of the vapour tube. Small quantities of toluol are allowed to boil for a few minutes in the boiler, and the copper-plate soon reaches the temperature of 107°-110°.

For the ordinary staining reagents (in watery fluids) it is enough to place the air-dried preparation at about 110° C. for one half to two minutes. For differential staining mixtures, for instance the eosin-aurantia-nigrosin mixture, a time of two hours is necessary, or higher temperatures must be employed.