Читать книгу Histology of the Blood, Normal and Pathological - Paul Ehrlich - Страница 9

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To obtain unexceptionable preparations cover-slips of particular quality are necessary. They should not be thicker than 0.08 to 0.10 mm., the glass must not be brittle or faulty, and must in this thickness easily allow of considerable bending, without breaking. Every unevenness of the slip renders it useless for our purposes. The glasses must previously be particularly carefully cleaned, and all fat removed. It is generally sufficient to allow the slips to remain in ether for about half-an-hour, not covering one another. Each one still wet with ether is then wiped with soft, not coarse, linen rag or with tissue-paper. The slips now are put into alcohol for a few minutes, are dried in the same manner as from the ether, and are kept ready for use in a dust-tight watch-glass. Bearing in mind, that these cover-slips are not cut out from a flat piece but from the surface of a sphere, it is evident that only with glasses thus prepared, can it be expected that a capillary space should be formed between two of them, in which the blood spreads easily. For with the smallest unevenness or brittleness of the glass it is an impossibility for the one to fit every bend of the other. And it is only then that the slips can be drawn away one from another, without using a force which breaks them.

To avoid fresh soiling of the cover-slips, and above all the contact of the blood with the moisture coming from the finger, the cover-glass is held with forceps[4] to receive the blood. We recommend for the under cover-glass a clamp forceps a, with broad, smooth blades; the ends may be covered with leather or blotting-paper for a distance of about ½ in. For the other cover-slip a very light spring forceps b, with smooth blades, sharp at the tips, is used, with which a cover-glass can be easily picked up from a flat surface. The lower slip is now fixed by one edge in the clamp forceps, and held ready in the left hand. The right hand applies the upper glass with the forceps b to the drop of blood as it exudes from the puncture, and takes it up, without touching the finger itself. The forceps b is then quickly brought to a and the slip with the little drop of blood allowed to fall lightly on the other. In glasses of the right quality the drop distributes itself spontaneously in a completely regular capillary layer. With two fingers of the right hand on the edge of the upper glass, it is now carefully pulled from the lower, which remains fixed in the clamp, without pressing or lifting. Frequently only one, the lower, shews a regular layer, but occasionally both are available for examination. During the desiccation in the air, generally complete in 10–30 seconds, the preparations must naturally be protected from any dampness (for example the breath of the patient).

The extent of surface which is covered depends on the size of the drop, the smaller the latter, the smaller the surface over which it has to be spread. Large drops are quite useless, for with them, the one cover-glass swims on the other, instead of adhering to it.

Although a written description of these manipulations makes the method seem rather intricate, yet but little practice is required to obtain an easy and sure mastery over it. We have felt compelled to describe the method minutely, since preparations so often come under our notice which, although made by scientific men, who pursue hæmatological investigations, are only to be described as technically completely inadequate.

The specimens so obtained, after they are completely dried in the air, should be kept between layers of filter-paper in well closed vessels till further treatment. In important cases, preparations of which it is desirable to keep for some considerable time, some of the specimens should be kept from atmospheric influences by covering them with a layer of paraffin. The paraffin must be removed by toluol before proceeding further. The preparations must naturally be kept in the dark.