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Table of Contents

Оглавление

Cover

Title Page

Copyright Page

Dedication Page

Biography

Acknowledgements Homo Sapiens – Part 1 Statistical Packages Homo Sapiens – Part 2

Foreword

1 Introduction Experimental design: the important decision about statistical analysis Statistical analysis: why are statistical tests required? The eye‐ball test! The structure of this book: Descriptive and Inferential Statistics

2 So, what are data? Data handling and presentation

10  3 Numbers; counting and measuring, precision, and accuracy Precision and accuracy Errors in measurement Independent observations or duplicate/triplicate/quadruplicate? That is the question!

11  4 Data collection: sampling and populations, different types of data, data distributions Sampling and populations The Central Limit Theorem Types of data Classification of data distributions So why do we need to understand data distribution?

12  5 Descriptive statistics; measures to describe and summarise data sets Parametric Descriptive Statistics and the Normal Distribution Example output from statistical software

13  6 Testing for normality and transforming skewed data sets Transforming skewed data sets to approximate a normal distribution Removing outliers: Grubbs's test QQ plots Example output from statistical software

14  7 The Standard Normal Distribution

15  8 Non‐parametric descriptive statistics Non‐parametric descriptive statistics Example output from statistical software

16  9 Summary of descriptive statistics: so, what values may I use to describe my data? Introduction: the most important question to answer in statistical analysis! What type of data do I have? Taking the first steps to data description and analysis Strategy for descriptive statistics Example data Example output from statistical software Decision Flowchart 1: Descriptive Statistics – Parametric v Non‐Parametric data

17  10 Introduction to inferential statistics Overview Hypothesis testing Experimental design

18  11 Comparing two sets of data – Independent t‐test The Independent t-test Equal group sizes Unequal group sizes Interpretation of the t statistic Example output from statistical software

19  12 Comparing two sets of data – Paired t‐test The Paired t-test Interpretation of the t statistic Example output from statistical software

20  13 Comparing two sets of data – independent non‐parametric data The Wilcoxon Rank Sum test and Mann-Whitney U-test The Wilcoxon Rank-Sum test The Mann–Whitney U‐test Example output from statistical software

21  14 Comparing two sets of data – paired non‐parametric data The Wilcoxon Signed‐Rank test Example output from statistical software

22  15 Parametric one‐way analysis of variance Introduction One‐Way Analysis of Variance Source of variance; Total, Between-Group, and Within-Group Relationship between the F‐ratio and probability So, what do we do next? Multiple pairwise comparisons; post hoc and a priori analysis Data analysis step 1: one‐way ANOVA Data analysis step 2: Post hoc analysis Example output from statistical software

23  16 Repeated measure analysis of variance Introduction Repeated measures ANOVA Assessing sphericity Mauchly's test Post hoc tests Example output from statistical software

24  17 Complex Analysis of Variance Models Part A: choice of suitable Analysis of Variance models. Using spreadsheets in experimental design Part B: choice of suitable post hoc pairwise comparisons Mixed ANOVA models Bonferroni and alternative correction procedures Holme correction procedure General comments on complex ANOVA models Example output from statistical software

25  18 Non‐parametric ANOVA Overview Limitations of non-parametric ANOVA models Multiple pairwise comparisons following non‐parametric ANOVA Multiple pairwise comparisons using the Mann–Whitney U‐test Multiple pairwise comparisons using the Wilcoxon Signed‐Rank test Multiple pairwise comparisons using a variant of Dunn's test Example output from statistical software

26  19 Correlation analysis Bivariate correlation analysis of parametric data Correlation analysis of non‐parametric data Example output from statistical software

27  20 Regression analysis Linear regression Example output from statistical software

28  21 Chi‐square analysis Assumptions of chi‐square analysis Risk, relative risk, and odds ratio Example output from statistical software Decision Flowchart 3: Inferential Statistics – Tests of Association

29  22 Confidence intervals Overview Statistical significance of confidence intervals

30  23 Permutation test of exact inference Rationale

31  24 General Linear Model The General Linear Model and Descriptive Statistics The General Linear Model and Inferential Statistics

32  Appendix A: Data distribution: probability mass function and probability density functions A.1 Binomial distribution (Chapter 4.iii, Figure 4.4): Probability mass function A.2 Exponential distribution (Chapter 4.v1, Figure 4.5): Probability density function A.3 Normal distribution (Chapter 4.vii, Figure 4.7): Probability density function A.4 Chi‐square distribution (Chapter 4.viii, Figure 4.8): Probability density function A.5 Student t‐distribution (Chapter 4.ix, Figure 4.9): Probability density function A.6 F distribution (Chapter 4.x, Figure 4.10): Probability density function

33  Appendix B: Standard normal probabilities

34  Appendix C: Critical values of the t‐distribution

35  Appendix D: Critical values of the Mann–Whitney U‐statistic

36  Appendix E: Critical values of the F distribution

37  Appendix F: Critical values of chi‐square distribution

38  Appendix G: Critical z values for multiple non‐parametric pairwise comparisons

39  Appendix H: Critical values of correlation coefficients Summary Decision Flowchart

40  Index

41  End User License Agreement

Experimental Design and Statistical Analysis for Pharmacology and the Biomedical Sciences

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