Читать книгу Essential Endocrinology and Diabetes - Richard I. G. Holt - Страница 92

Some metabolites are assayed enzymatically, frequently using dye substrates that are catalyzed to products that are coloured or fluoresce. By incorporating known standards, the amount of colour or fluorescence can be used for precise quantification. For example, glycated haemoglobin (HbA_1c), a measure of long‐term diabetes control (Chapter 11) can be measured in an enzymatic assay as well as by immunoassay and chromatography/MS approaches. Serum glucose can be measured by oxidation to generate a product that interacts with a dye to generate colour or fluorescence in an enzymatic assay.