Читать книгу Principles of Virology, Volume 2 - Jane Flint, S. Jane Flint - Страница 25

A new approach for determining the sequence of a nucleic acid has been developed, referred to as “nanopore sequencing.” This method relies on the use of biological nanopores, such as the bacterial hemolysin, which forms extremely small holes, or pores, in a membrane. These pores have a diameter wide enough to allow only a single strand of RNA or DNA to pass through. When an ionic current is applied to the membrane, each of the four nucleotides passing through the pore alters the current in a characteristic manner, which can be interpreted by a sensor (yellow starburst in panel A in the figure) and de coded to provide the sequence. This approach obviates the need for PCR amplification, greatly reducing experimental error that can accompany other sequencing techniques that rely on such amplification. Moreover, this approach is portable to remote locations, accelerating pathogen identification at sites of outbreaks.

 Kafetzopoulou LE, et al. 2019. Metagenomic sequencing at the epicenter of the Nigeria 2018 Lassa fever outbreak. Science 363:74–77.

Simplified overview of the process of nanopore sequencing.