Читать книгу Secondary Metabolites of Medicinal Plants - Bharat Singh - Страница 56

The callus cultures were established with different growth regulators (benzylaminopurine (BAP), naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA); 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine) in B. pinnatum (Gurnani et al. 2014). The cultures were incubated in the darkness at 24 ± 1 °C for 50 days. The percentage of callus induction and the area of explants covered by callus cells were evaluated. The highest percentage of callus induction was 100%, obtained with the combination of 2,4-D and benzyl adenine (BA) (Santos et al. 2014).