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Enzymes are the third key component in understanding how the endocannabinoid system encourages balance. The enzymes are proteins that speed up chemical reactions. They help produce and break down endocannabinoids in the body.

Other enzymes transport ECS-specific proteins. Endocannabinoids are fat-soluble, and the human body is composed primarily of water. Therefore, the endocannabinoids need an enzyme to move them to the area of interest. These enzymes react to periods of imbalance; when the balance is restored, other enzymes break down the endocannabinoids, and business as usual resumes.