Читать книгу Chemical Analysis - Francis Rouessac - Страница 52

Equation (1.42) highlights the need to determine peak areas. To do so, chromatography software has been developed to ensure control of the chromatogram, the acquisition of chromatograms and the determination of certain information related to the observed peaks, such as the FWHM δ, the width at the base ω, and the peak height or its area. These software programs help correct the baseline, select the start and end, if necessary, of each peak for integration, and, most importantly for quantitative analysis, select the quantification method. Lastly, by taking into account compositions of reference solutions (calibration solutions), they calculate sample compositions.

The three quantification methods used in chromatography (and sometimes in other techniques using proportionality between composition and response of a measuring instrument) are described here.