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This second method relies on the relative response factor of each compound to be measured against a marker added as a reference. This compensates for any imprecision in the injected volumes, which is the main drawback of the previous method.

As above, this more reliable method requires two chromatograms, one to calculate the relative response factors of the compounds of interest, and the other to analyse the sample.

The areas of the peaks to be quantified are compared with that of an internal standard (designated by IS), added at a known concentration to the sample solution.