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North carolina design II

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In this design, each member of a group of parents used as males is mated to each member of another group of parents used as females. Design II is a factorial mating scheme similar to Design I (Figure 4.6). It is used to evaluate inbred lines for combining ability. The design is most adapted to plants that have multiple flowers so that each plant can be used repeatedly as both male and female. Blocking is used in this design to allow all the mating involving a single group of males to a single group of females to be kept intact as a unit. The design is essentially a 2‐way ANOVA in which the variation may be partitioned into difference between males and females and their interaction. The ANOVA is as follows:


Figure 4.6 North Carolina Design II. (a) This is a factorial design. (b) Paired rows may be used in the nursery for factorial mating of plants.

Source df MS EMS
Males n1 – 1 MS1 σ2w + rσ2mf + rnσ2m
Females n2 – 1 MS2 σ2w + rσ2mf + rn1σ2f
Males × females (n1 – 1)(n2 – 1) MS3 σ2w + rσ2mf
Within progenies n1n2(r − 1) MS4 σ2w




The design also allows the breeder to measure not only GCA but also SCA.

Principles of Plant Genetics and Breeding

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