Читать книгу Biotechnology of Fruit and Nut Crops - Группа авторов - Страница 173

Carlier et al. (2004) published the first genetic map of pineapple, which was constructed using 46 F1 individuals from the cross between A. comosus var. comosus and A. comosus var. bracteatus. The var. bracteatus map consisted of 335 markers: 60 RAPD, 264 AFLP and 11 inter-simple-sequence repeat (ISSR) markers. It contained 50 linkage groups that span 3693 cM. The var. comosus map consisted of 157 markers, including markers for the piping trait, and contained 30 linkage groups that span 4146 cM.

Carlier et al. (2012) later constructed a genetic map of pineapple using an F2 population from a single selfed F1 plant produced by a cross between var. comosus and var. bracteatus. The map included 492 DNA markers: 57 RAPD, 22 ISSR, 348 AFLP, 20 SSR, 12 EST-SSR, 25 sequence-characterized amplified region and 8 cleaved amplified polymorphic sequence (CAPS) markers in 33 linkage groups. de Sousa et al. (2013) combined previous published maps of F1 and F2 progeny to produce a new genetic map that included 741 marker loci, which were integrated into 28 linkage groups that spanned 2113 cM and covered 86% of the genome.

Nashima et al. (2016) generated 55,621 SSRs with di-, tri-, tetra-, penta- and hexanucleotide motifs using the 454 GS FLX+ platform. SSR markers designed from the newly obtained genome sequence were evaluated for alleles per locus and genetic diversity and used to construct a genetic linkage map. Fang et al. (2016) conducted a detailed characterization of microsatellites in pineapple and identified 278,245 SSRs and 41,962 SSRs with an overall density of 728.57 SSRs/Mb and 619.37 SSRs/Mb in genomic and EST sequences, respectively. This was unexpectedly high given the moderate size of the pineapple genome. Arumuganathan and Earle (1991) estimated the haploid genome size at 526 Mbp for var. comosus and 444 Mbp for var. bracteatus.