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This method is particularly suitable for collagen‐based scaffolds. This is because the collagen has a higher sensitivity to heat denaturation and chemicals. It has been found that aortic heart valve cells can be grown by creating these porous collagen scaffolds [26]. For entrapping the ice crystals, these collagen type 1 fibers were frozen at −30 °C. The required porous materials can be obtained by removing the crystals using a process known as lyophilization. The prominent parameters that affect the pore size include temperature, pH, and solution concentration. Emulsion freeze drying method is used for producing scaffolds having heat‐sensitive bioactive molecules with pore sizes in the range 20–200 μm and with porosity greater than 90% [27, 28].