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 Option 1. Collect a swab sample of the endometrium and uterine lumen using traditional techniques (see Chapter 12). The swab tip is placed inside a sterile 15 ml centrifuge tube and submitted for qPCR analysis. The sample should be kept refrigerated or shipped on ice during transport to the laboratory to help maintain DNA integrity. Freezing or excessive heat should be avoided.

 Option 2. Collect a low volume uterine lavage sample using traditional techniques (see Chapter 18). Aliquot approximately equal volumes of the recovered effluent fluid into four separate 15 ml centrifuge tubes. The tubes are centrifuged at 600× g for 10 minutes. The supernatant is discarded and the residual pellets remaining at the bottom of the centrifuge tube are submitted for qPCR analysis, traditional microbial culture, and cytologic analysis. The pellet in the last centrifuge tube can be held for later analysis as needed.