Читать книгу Veterinary Surgical Oncology - Группа авторов - Страница 35

Small biopsy samples should be placed in fixative immediately to prevent drying of the sample. Early fixation will initiate changes in the sample that will prevent autolysis and bacterial alteration of the sample (Stevens et al. 1974). In large biopsy submissions, the sample should be sliced evenly to allow for more complete fixation (Dernell and Withrow 1998; Ehrhart and Powers 2007). However, when slicing a large specimen, care should be taken not to slice through the surgical margins but rather leave those untouched. Therefore, the slicing is done through the skin into the tumor for cutaneous or subcutaneous tumors. Many fixatives including formalin, Bouin’s fluid, chilled isopentane, Zenker’s fluid, and glutaraldehyde have been described in veterinary medicine (Osborne 1974; Stevens et al. 1974), but in general, 10% buffered formalin is sufficient for almost all biopsies. A biopsy sample should be fixed in formalin in a 1:10 solution of tissue to formalin (Ehrhart and Withrow 2007).