Читать книгу Genotyping by Sequencing for Crop Improvement - Группа авторов - Страница 19

Sequence‐tagged sites (STSs) were first developed for physical mapping of the human genome by Olsen et al. (1989). STS is the short unique sequences developed from polymorphic RFLP probe or AFLP fragment which is linked to desirable traits. The RFLP probes or AFLP fragments showing polymorphism are end‐sequenced and primers are designed to specifically amplify these fragments. STS markers are co‐dominant and highly reproducible. For example, STS markers have been developed for RFLP markers linked with bacterial blight resistance genes xa5, xa13, and Xa21 (Huang et al. 1997). One major limitation of these types of markers is the reduced polymorphism than the corresponding RFLP probe.