Читать книгу Genotyping by Sequencing for Crop Improvement - Группа авторов - Страница 21

These markers overcome the limitation of RAPDs. In this case, the RAPD fragments that are linked to a gene of interest are cloned and sequenced. Based on the terminal sequences, longer primers (20 mer) are designed. These SCAR primers more specifically amplify a particular locus. These are similar to STS markers in design and application. The presence or absence of the band indicates variation in sequences. The SCAR markers thus are dominant in nature. These, however, can be converted to codominant markers in certain cases by digesting the amplified fragment with tetranucleotide recognizing restriction enzymes. There are several examples where the RAPD markers linked to the gene of importance have been converted to SCAR markers (Joshi et al. 1999; Liu et al. 1999; Kasai et al. 2000; Akkurt et al. 2007; Chao et al. 2018).